human pancreatic carcinoma cell lines panc1  (ATCC)

Journal: PLoS ONE

Article Title: Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles

doi: 10.1371/journal.pone.0151339

Figure Lengend Snippet: Panel A. TGF-beta induces epithelial to mesenchymal transition (EMT) in PANC1 cells . Real Time PCR analysis showed a decrease of the epithelial marker E-cadherin (p-v<0.05) and an increase of the mesenchymal markers N-Cadherin and Vimentin (p-v<0.05) expression after TGF-β treatment in PANC1 cells. Panel B . Principal Component Analysis (PCA) of exosomal metabolome for MS negative ionization mode showing the separation between the two study groups . TGF-β treated (T) and control (C) PANC1 cells. We analyzed three replicates per condition. The x-axis shows interclass separation while y-axis illustrates the intra-class variability on Y-axis.

Article Snippet: Human pancreatic carcinoma epithelial-like cell line PANC1 (ATCC ® CRL-1469TM) was obtained from the Tissue Culture Shared Resource of Georgetown University (Washington DC, USA).

Techniques: Real-time Polymerase Chain Reaction, Marker, Expressing, Control

Journal: British Journal of Cancer

Article Title: Polypeptide N -acetylgalactosaminyltransferase 6 expression in pancreatic cancer is an independent prognostic factor indicating better overall survival

doi: 10.1038/bjc.2011.166

Figure Lengend Snippet: Immunohistochemical and western blotting analyses of GalNAc-T6 expressions in well-differentiated pancreatic cancer sample ( × 200) and PANC1 cell line after incubation of an anti-GalNAc-T6 polyclonal antibody with or without synthesised peptides of GalNAc-T6. IHC, immunohistochemistry; WB, western blotting. Bar, 100 μ m. GalNAc-T6 expression is completely abolished with peptide.

Article Snippet: Human pancreatic invasive ductal carcinoma cell lines PANC1 (American Type Culture Collection) and PK1 (a kind gift from the Institute of Development, Aging and Cancer, Tohoku University, Japan) were maintained in a DMEM medium containing 10% fetal calf serum (GIBCO BRL, Rockville, MD, USA) at 37°C in an atmosphere of 95% air and 5% CO 2 .

Techniques: Immunohistochemical staining, Western Blot, Incubation, Immunohistochemistry, Expressing

Journal: British Journal of Cancer

Article Title: Polypeptide N -acetylgalactosaminyltransferase 6 expression in pancreatic cancer is an independent prognostic factor indicating better overall survival

doi: 10.1038/bjc.2011.166

Figure Lengend Snippet: Immunofluorescent and western blotting analyses of GalNAc-T6 in pancreatic cancer cell lines PANC1 and PK1. IF, immunofluorescence. WB, western blotting. The cytoplasmic expression levels of GalNAc-T6 are significantly higher in primary cancer cell line PANC1 than those in metastatic cancer cell line PK1 with both methods.

Article Snippet: Human pancreatic invasive ductal carcinoma cell lines PANC1 (American Type Culture Collection) and PK1 (a kind gift from the Institute of Development, Aging and Cancer, Tohoku University, Japan) were maintained in a DMEM medium containing 10% fetal calf serum (GIBCO BRL, Rockville, MD, USA) at 37°C in an atmosphere of 95% air and 5% CO 2 .

Techniques: Western Blot, Immunofluorescence, Expressing